Spanish PM Jose Luis Rodriguez Zapatero said Spain would demand reparations for the economic losses suffered.
The outbreak, centred on Germany, has been caused by a new form of the E. coli bacterium, health experts say. Seven people in the UK have the infection. They are all thought to have contracted it in Germany.
Spanish fruit and vegetable exporters estimate they are losing 200m euros ($290m; £177m) a week in sales after Germany said earlier in the outbreak that it probably originated with Spanish cucumbers.
No evidence of this has been found and researchers are scrambling to find the source.
Health advice
- Wash fruit and vegetables before eating them
- Peel or cook fruit and vegetables
- Wash hands regularly to prevent person-to-person spread of E. coli strain
"We acted as we had to, and we are going to get reparations and the return of Spanish products to their rightful place," said Spanish Prime Minister Zapatero.
"I believe that any other interpretation or any effort to politicise the huge mistake made by the German authorities is totally unfair."
Tens of thousands of kilos of fresh fruit and vegetables grown in Spain are being destroyed, says the BBC's Sarah Rainsford in Almeira, Spain's "fruitbasket".
Sales to supermarkets across Europe have ground to a halt, our correspondent says: not just of cucumbers, but of everything.
The European Union has urged Russia - its largest export market for vegetables - to drop its ban on the import of fresh vegetables, describing the move as totally disproportionate.
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